Queensland Health believes that the nurse, who resides in North Brisbane, then transmitted the infection to her partner, who spread the infection to his social network.
For the next two weeks, all Queenslanders will be required to carry a mask when they leave their home and to wear them in indoor spaces.
The man also transferred Covid to a doctor, Queensland Chief Health Officer Jeannette Young said.
In broader measures, gatherings at homes across the state will remain capped at 30, dancing in public venues is banned and businesses must maintain distancing of one person per two square metres.
Ms Palaszczuk said the one new case of community transmission had tested negative in self-isolation on Monday night before returning a positive result on Wednesday.
There were 34,7111 test in the past 24 hours to this morning.
"Which is what we know COVID does".
Since the call was made, a "large number" of staff have been asked to self-isolate in a bid to halt the spread.
Any workers who set foot inside the ward from March 23 to 26 have also been ordered into two weeks of quarantine, even if they weren't directly involved in patient care.
People quarantining in the ACT were being advised directly by ACT Health about what to do next.
She is a nurse who became infected with Covid after treating a "superspreader" who arrived from Europe last month.
A second cluster linked to a junior doctor who contracted the virus in early March sits at eight cases.
She said public health requirements will remain in place for anyone who had been in identified exposure sites, with anyone who has been in Greater Brisbane or the Byron Shire in NSW in the last 14 days urged to remain vigilant for symptoms.
The virus scare in Byron Bay prompted the cancellation of the tourist town's Bluesfest music festival and the reintroduction of restrictions in northern NSW.
Infectious cases have visited venues in Greater Brisbane, the Gold Coast, Gladstone, Toowoomba, Hervey Bay and Gin Gin.
Anyone at the pub at the same time is considered a close contact and must self-isolate for 14 days and get tested immediately.
The following locations have been listed by Queensland Health as casual contacts.
As Greater Brisbane residents scramble to revive their plans for the weekend, health authorities are urging people to be cautious.
'We are confident that New South Wales is able to deal with the current scare, Premier Gladys Berejiklian told reporters.