Transport officials have said services on the Docklands Light Railway are delayed, following the protest action.
Police switched off the Tube network's Wi-Fi connection this morning to deter climate change protesters who are planning to disrupt London's transport network.
Groups of protesters remained in place through the night and into Wednesday morning at several locations, blocking the road at Marble Arch and Oxford Circus.
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The stunt comes on the third day of a series of climate change protests which have led to almost 300 people being arrested in the capital.
Protesters stand behind their banner blocking a road near Parliament Square in central London.
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In a statement posted on Twitter he said: "It is absolutely crucial to get more people using public transport, as well as walking and cycling, if we are to tackle this climate emergency - and millions of Londoners depend on the Underground network to get about their daily lives in our city".
Passengers were not informed about the decision to turn off the network, and many travellers wrongly attributed the disruption to conventional technical faults.
"During the next 24 hours, these intelligence-led patrols will be in place to help keep everyone safe and to ensure that disruption is kept to a minimum".
"Targeting public transport in this way would only damage the cause of all of us who want to tackle climate change", he said in a statement. In The Hague on Tuesday, activists occupied the global criminal court building.
Elsewhere on Tuesday, 29 people were arrested after hundreds of activists from the Extinction Rebellion Scotland group occupied Edinburgh's North Bridge, bringing traffic to a standstill. By 6am on Wednesday, all had been released and charged with breach of the peace, to appear in court at a later date.
The Metropolitan Police said 500,000 people had been affected by the diversion of bus routes.
A Transport for London Spokesman also said that it was aware of the planned demonstrations and advised travels to check for travel updates.
The police have ordered the protesters to confine themselves to a zone within Marble Arch, a space at the junction of Hyde Park, the Oxford Street main shopping thoroughfare and the Park Lane street of plush hotels.
Police said they expected the demonstrations to continue in the next few weeks and promised to take action if necessary. It is calling on the United Kingdom government to reduce carbon emissions to zero by 2025 and establish a citizens' assembly to devise an emergency plan of action to tackle climate breakdown and biodiversity loss.