The president added that "The problem is that Emmanuel suffers from a very low Approval Rating in France, 26%, and an unemployment rate of nearly 10%".
Macron's office said it refused to comment on any of Trump's tweets criticising the French president.
"The problem is that Emmanuel suffers from a very low Approval Rating in France", tweeted Trump, who is something of an expert in low approval ratings.
Mr Trump's continued complaints about France and Macron struck a nerve with some in the European nation, especially since the president's latest broadsides came on the third anniversary of the Paris terror attacks that killed 130 people in 2015.
Donald Trump has once again accused France of wanting to build a European army to protect itself from the U.S. after apparently misunderstanding Emmanuel Macron for the second time in less than a week.
President Donald Trump is slamming French President Emmanuel Macron and pushing back against criticism he faced over the weekend for skipping a memorial marking the 100th anniversary of the end of the First World War.
It marks a turnaround in the pair's relationship, which was once more of a "bromance" when Macron visited the U.S.in April - where the two leaders held hands, referred to each other affectionately (Trump described the dashing Frenchman as "perfect") and shared awkward hugs.
In his first tweet on Friday, sent while on Air Force One, Trump said he found Macron's comments "very insulting" and said France should "first pay its fair share of Nato".
"By the way, there is no country more Nationalist than France, very proud people-and rightfully so!" the US leader tweeted.
"MAKE FRANCE GREAT AGAIN!" he added.
White House Director of Strategic Communications Mercedes Schlapp, meanwhile, cast Trump's Paris trip in a positive light.
"It was clearly a successful trip", Schlapp told Fox News on Tuesday, saying Trump and Macron "had a productive meeting" on trade and North Atlantic Treaty Organisation.
Having increased his clout by concluding the Idlib cease-fire agreement, hosting the Four-Nation Summit on the situation in Syria, and skillfully responding to the murder of Saudi journalist Jamal Khashoggi, President Recep Tayyip Erdoğan sent a number of powerful messages to the global public in an op-ed essay for Le Figaro.
Within just an hour of making that comment, Putin told other reporters that he did in fact briefly meet with Mr. Trump.
THE ASSOCIATED PRESS French President Emmanuel Macron's wife, Brigitte Macron (center left) listens to Canadian Prime Minister Justin Trudeau (center right) as they walk with world leaders toward the Arc de Triomphe in Paris on Sunday.
Trump on Sunday also missed a procession of world leaders who somberly walked shoulder-to-shoulder in the rain as bells tolled marking the exact moment the war's fighting ended: 11 a.m. on November 11, 1918. Shortly before the commemorations began, Macron touted the idea of creating a European army against global threats including the United States.
The weekend started uncomfortably.
Earlier Tuesday, Trump took aim at France's war record in response to Macron last week suggesting Europe needed an army to "protect ourselves with respect to China, Russia, and even the United States of America".
Mr Trump said: "We want a strong Europe, it's very important to us and whichever way we can do it the best and more efficient would be something we both want".